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Fluid Enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All Fluid Enzymes show superior activity in the universal CutOne® and CutOne® Colour Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. Fluid Enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Colour Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Colour Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
Components
| Component | Amount |
|---|---|
| AatII (20 U/μl) | 30 μl (600 U) |
| 10× CutOne® Buffer | 1 ml |
| 10× CutOne® Colour Buffer | 1 ml |
Recommended Reaction Conditions
1× CutOne® Buffer；
Incubate at 37℃；
Refer to “Protocol for Fast DNA Digestion” for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Storage Condition
Store at -20℃ for 2 years.
