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All-in-One First-Strand Synthesis MasterMix is a high-quality, efficient, and convenient one-step cDNA synthesis mix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo(dT)20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Using the reverse transcription pre-mix, cDNA of up to 12 kb in size can be obtained within 15 minutes.
Components
| Component | Amount |
|---|---|
| All-in-One First-Strand Synthesis MasterMix | 400 μl |
| Nuclease-Free Water | 2×1 ml |
Storage Condition
-20℃
