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BsmBI is a Type IIS restriction enzyme that recognizes non-palindromic sequences and cuts outside the recognition site. It is commonly used in Golden Gate assembly. The reaction buffer has been optimized to maximize the functionality of BsmBI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.
Components
| Component | Amount |
|---|---|
| BsmBI (10 U/μl) | 20 μl |
| 10× Cut Buffer C | 1 ml |
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 55℃；
Refer to “Protocol for DNA Digestion” for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.
Storage Condition
-20℃
