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Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5′ → 3′ exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli, this enzyme has been engineered. It possesses robust 5′ → 3′ DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.
Components
| Component | Amount |
|---|---|
| Bst II DNA Polymerase (Large Fragment) (8 U/μl) | 1 ml |
| 10× Bst II Reaction Buffer | 3×1 ml |
| MgSO4 (100 mM) | 3×1 ml |
Storage Condition
-20℃
