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Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the Nε-crotonyl-L-lysine (Kcr) into the target protein by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical amino acid Kcr, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements.
Note: This product under this catalog number enables the expression of disulfide bond-rich proteins.
Components
| Component | Amount |
|---|---|
| Cell-free system solution A | 300 μl |
| Cell-free system solution B | 400 μl |
| CFPS-Control Plasmid | 2 μg |
| Nε-crotonyl-L-lysine Kcr (25×) | 60 μl |
| tRNA (25×) | 60 μl |
| Aminoacyl-tRNA synthetase (50×) | 40 μl |
Storage Condition
Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.
To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.
