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High T7 RNA Polymerase is a genetically engineered thermostable T7 RNA polymerase, which exhibits high specificity for the bacteriophage T7 promoter sequence. Compared to the wild-type T7 RNA polymerase, High T7 RNA Polymerase enables efficient in vitro transcription (IVT) at temperature from 37 to 52℃.
Components
| Component | Amount |
|---|---|
| High T7 RNA Polymerase(50 U/μl) | 500 μl |
| 10× T7 RNA Pol Buffer | 1.25 ml |
Applications
1. Synthesis of single-stranded RNA, including mRNA, siRNA, gRNA, and other types of RNA precursors.
2. Synthesis of labeled or unlabeled highly specific RNA probes.
3. Synthesis of capped mRNA using cap analogs.
Storage Condition
-20℃
