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SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5′-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM.
SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.
Components
| Component | Amount |
|---|---|
| SpCas9-NLS (10 μM) | 10 μl |
| 10× Cut Buffer C | 1 ml |
Storage Condition
Store at -20℃ for 2 years.
