Taq-Plus PCR Forest Mix (2×) V2 is a pre-mixed PCR reaction solution formulated with Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up.
It is capable of robust amplification of up to 6 kb from genomic DNA. PCR products amplified with this mix have a 3′-terminal adenosine (A), and therefore PCR products can be used directly for T/A cloning.
The Forest mix is supplemented with two tracking dyes that allows for direct loading of the PCR product on a gel. The dyes in the mix do not interfere with PCR performance but need to purify the PCR product when it’s used for absorbance, fluorescence, etc. In addition, the dye-free Taq-Plus PCR Master Mix (2×) V2 can also be used.
Components
| Component | Amount |
|---|---|
| Taq-Plus PCR Forest Mix (2×) V2 | 5×1 ml |
Storage Condition
-20℃

