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Tris-EDTA (TE) appears as white instant granules. Each pouch makes 1 L of 1x TE solution conveniently with a simple procedure. 1× TE buffer contains 10 mM Tris-HCl and 1 mM EDTA, with pH 8.0 at 25°C.
TE buffer is commonly used as a solvent or preservative to store and dilute DNA or RNA.
Components
| Component | Amount |
|---|---|
| Tris-EDTA (pH 8.0) | 100 Pouches |
Note: pH is 8.0±0.1@25℃ when made to 1× solution.
Protocol
1. Put magnetic stirring beads and ~600 ml distilled water into a beaker.
2. While stirring, slowly pour the whole contents from 1 pouch of TE into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1× solution is made.
Note: When TE buffers are used to perform DNA or RNA related work, it is recommended to use nuclease-free water for preparation.
Storage Condition
Room temperature
