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phi29 DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. phi29 DNA Polymerase exhibits a strong strand-displacement activity, a strong chain affinity, and a single polymerization can achieve a continuous polymerization extension up to 70 kb. In addition, phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5′ exonuclease activity. However, because of the enzymes’s 3’→5′ proofreading exonuclease, 3′-modified primers are highly recommended.
Components
| Component | Amount |
|---|---|
| phi29 DNA Polymerase (10 U/μl) | 50 μl |
| 10× phi29 Buffer | 1 ml |
Storage Condition
Store at -20℃ for 2 years.
