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This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BsaI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BsaI present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for BsaI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BsaI bound in the initial step. The color development is stopped and the intensity of the color is measured. And the residual degree of BsaI in the sample is measured.
Detection range: 0.188~12 ng/ml
Quantification limit: 0.188 ng/ml
Detection limit: 0.094 ng/ml
Components
| Component | Amount |
|---|---|
| BsaI Standard | 10 μg/ml, 0.8 ml, 1 Vial |
| BsaI Detection Antibody (HRP labeled) | 12 ml, 1 Vial |
| TMB Substrate | 12 ml, 1 Vial |
| Stop Solution | 12 ml, 1 Vial |
| Sample Diluent | 50 ml, 1 Vial |
| Wash Buffer (20×) | 50 ml, 1 Vial |
| Microtiter Plate (Coated) | 96-well, 1 Plate |
Storage Condition
Stored at 2~8℃ . Keep away from light.
