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This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for XhoI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XhoI present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for XhoI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XhoI bound in the initial step. The color development is stopped and the intensity of the color is measured. And the residual degree of XhoI in the sample is measured.
Detection range: 0.125~4 ng/ml
Quantification limit: 0.125 ng/ml
Detection limit: 0.021 ng/ml
Components
| Component | Amount |
|---|---|
| XhoI Standard | 10 μg, 2 Vial |
| XhoI Detection Antibody (HRP labeled) | 12 ml, 1 Vial |
| TMB Substrate | 12 ml, 1 Vial |
| Stop Solution | 12 ml, 1 Vial |
| Sample Diluent | 50 ml, 1 Vial |
| Wash Buffer (20×) | 50 ml, 1 Vial |
| Microtiter Plate (Coated) | 96-well, 1 Plate |
Storage Condition
Stored at 2~8℃. Keep away from light.
